Thursday, 30 August 2012
Image processing using Fiji ImageJ and Adobe Photoshop / Illustrator
This is my workflow for processing SEM or TEM images to make them publication ready.
Images from SEM are usually saved as tif files and come in two versions. One contains the scale bar and other information, the other one does not.
I want to get the scale bar from one image and put it into the empty one:
In Fij, I open both images and use set scale to extract the scale from the first image. I then set the same scale in the second image, and input a scale bar using Analyze -> Tools -> Scale bar.
Then I open the image in photoshop and crop it to size (8.25cm width, 600dpi) and turn it into grayscale.
Then I open the image in Illustrator and insert a scale bar (2mm high), with writing at 10pt, bold and Myriad Pro.
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